A COMPARATIVE STUDY OF ICT STOOL ANTIGEN, SERUM AND ELISA TECHNIQUES IN DETECTION OF HELICOBACTER PYLORI AMONG SUDANESE FOOD HANDLER

Mohammed Shams Alfalah; Abdelhafez Awad Elkarim Ahmed; Yousif Abdelhameed Mohammed; Albadawi Abdelbagi Talha

A COMPARATIVE STUDY OF ICT STOOL ANTIGEN, SERUM AND ELISA TECHNIQUES IN DETECTION OF HELICOBACTER PYLORI AMONG SUDANESE FOOD HANDLER

Mohammed Shams Alfalah,

Abdelhafez Awad Elkarim Ahmed,

Yousif Abdelhameed Mohammed,

Albadawi Abdelbagi Talha

Annotatsiya

Background: Helicobacter pylori (H. pylori) remains one of the most common human infections in Sudan recently and is associated with a number of important chronic gastritis, peptic ulcer disease and gastric malignancy. Objectives: The aim of this study was to compare the detection of H. pylori IgG in serum using ELISA techniques compared to ICT blood test and stool antigen. Materials and Methods: Stool and blood specimens were collected from 100 patients (mean age 31.2 ± 11.7 years, 56% males). Stool samples were analyzed using rapid stool antigen test for H. pylori and Serum samples were analyzed for H. pylori IgG by Accurate© (USA) ELISA and ICT blood test. Data analysis was made by the software of the Statistical Package for Social Sciences (SPSS) program (version 22). Results: The incidence of H. pylori among male was 12/17 (71%) compare to 5/17 (29%) females. 17 (17%) patients have positive with rapidstoolAg ICT test compare to 20 (20%) patients have positive by H. pylori IgG ELISA [the Accurate© (USA)]. The sensitivity, specificity, positive predictive value and negative predictive value for H. pylori IgG ELISA were (100%, 96.1%, 93.3% and 100% respectively) compared to ICTH. pylori IgG for blood (41.18%, 71.08%, 43.4% and 69.2% respectively) using rapid stool Ag ICT test as gold standard method.  Conclusion:  The  better results Sensitivity and Specificity obtained for H. Pylori diagnosis wasH. pylori IgG using ELISA techniques compared to ICT blood test.

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